School of Medicine

Wayne State University School of Medicine


Yedidi RS, Liu Z, Kovari IA, Woster PM, Kovari LC. P1 and P1' para-fluoro phenyl groups show enhanced binding and favorable predicted pharmacological properties: Structure-based virtual screening of extended lopinavir analogs against multi-drug resistant HIV-1 protease. J Mol Graph Model. 2014 Feb;47:18-24. doi: 10.1016/j.jmgm.2013.10.010. Epub 2013 Nov 1.


Kovari LC, Brunzelle JS, Lewis KT, Cho WJ, Lee JS, Taatjes DJ, Jena BP. X-ray solution structure of the native neuronal porosome-synaptic vesicle complex: Implication in neurotransmitter release. Micron. 2014 Jan;56:37-43. doi: 10.1016/j.micron.2013.10.002. Epub 2013 Oct 16.


Dewdney TG, Wang Y, Liu Z, Sharma SK, Reiter SJ, Brunzelle JS, Kovari IA, Woster PM, Kovari LC. Ligand modifications to reduce the relative resistance of multi-drug resistant HIV-1 protease. Bioorg Med Chem. 2013 Dec 1;21(23):7430-4. doi: 10.1016/j.bmc.2013.09.045. Epub 2013 Sep 27.


Dewdney, T.G., Wang, Y., Kovari I.A., Reiter, S.J., Kovari, L.C. 2013. Reduced HIV-1 integrase flexibility as a mechanism for raltegravir resistance. J Struct Biol. 2013 Nov;184(2):245-50. doi: 10.1016/j.jsb.2013.07.008. Epub 2013 Jul 25.


Yedidi, R.S., Muhuhi, J.M., Liu, Z., Bencze, K.Z., Koupparis, K.M., O'Connor, C.E., Kovari, I.A., Spaller, M.R., Kovari, L.C. 2013. Design, synthesis and evaluation of a potent substrate-analog peptide-inhibitor identified by scanning Ale/Phe chemical mutagenesis, mimicking substrate co-evolution, against multidrug-resistant HIV-1 protease. Biochem Biophys Res Commun. 2013 Sep 6;438(4):703-8. doi: 10.1016/j.bbrc.2013.07.117. Epub 2013 Aug 3.


Liu, Z.,  Yedidi, R., Wang, Y., Dewdney, T.G., Reiter, S.J., Brunzelle, J.S., Kovari, I.A., Kovari, L.C. 2013. Crystallographic study of multi-drug resistant HIV-1 protease lopinavir complex: Mechanism of drug recognition and resistance. Biochem Biophys Res Commun. 2013 Jul 26;437(2):199-204. doi: 10.1016/j.bbrc.2013.06.027. Epub 2013 Jun 18.


Liu Z, Yedidi RS, Wang Y, Dewdney TG, Reiter SJ, Brunzelle JS, Kovari IA, Kovari LC. 2013. Insights into the mechanism of drug resistance: X-ray structure analysis of multi-drug resistant HIV-1 protease ritonavir complex. Biochem Biophys Res Commun. 2013 Feb 8;431(2):232-8. doi: 10.1016/j.bbrc.2012.12.127. Epub 2013 Jan 8.


Liu, Z., Wang, Y., Yedidi, R., Dewdney, T.G., Reiter, S.J., Brunzelle, J.S., Kovari, I.A., Kovari, L.C. 2013. Conserved hydrogen bonds and water molecules in MDR HIV-1 protease substrate complexes. Biochem Biophys Res Commun. 2013 Jan 18;430(3):1022-7. doi: 10.1016/j.bbrc.2012.12.045. Epub 2012 Dec 19.


Wang, Y., Dewdney, T.G., Liu, Z., Reiter, S.J., Brunzelle, J., Kovari, I.A., Kovari L.C. 2012. Higher desolvation energy reduces molecular recognition in multi-drug resistant HIV-1 protease. Biology 1(1), 81-93; doi:10.3390/biology1010081


Yedidi RS, Liu Z, Wang Y, Brunzelle JS, Kovari IA, Woster PM, L.C. Kovari, Gupta D. 2012. Crystal structures of multidrug-resistant HIV-1 protease in complex with two potent anti-malarial compounds. Biochem Biophys Res Commun. 2012 May 11;421(3):413-7. doi: 10.1016/j.bbrc.2012.03.096. Epub 2012 Mar 24.


Liu Z, Wang Y, Yedidi RS, Brunzelle JS, Kovari IA, Sohi J, Kamholz J, Kovari LC. 2012. Crystal structure of the extracellular domain of human myelin protein zero. Proteins. 2012 Jan;80(1):307-13. doi: 10.1002/prot.23164. Epub 2011 Oct 4.


Wang, Y., Dewdney, T.G., Liu, Z., Reiter S.J., Brunzelle, J.S., Kovari, I.A., Kovari L.C. 2011. X-ray crystal structure and dynamics reveal HIV-1 protease drug interactions.Studia UBB Chemia.


Wang Y, Liu Z, Brunzelle JS, Kovari IA, Dewdney TG, Reiter SJ, Kovari LC. 2011. The higher barrier of darunavir and tipranavir resistance for HIV-1 protease. Biochem Biophys Res Commun. 2011 Sep 9;412(4):737-42. doi: 10.1016/j.bbrc.2011.08.045. Epub 2011 Aug 17.


Yedidi RS, Proteasa G, Martinez JL, Vickrey JF, Martin PD, Wawrzak Z, Liu Z, Kovari IA, Kovari LC. 2011. Contribution of the 80s loop of HIV-1 protease to the multidrug-resistance mechanism: crystallographic studies of MDR769 HIV-1 protease variants. Acta Crystallogr D Biol Crystallogr. 2011 Jun;67(Pt 6):524-32. doi: 10.1107/S0907444911011541.



Liu Z, Wang Y, Brunzelle J, Kovari IA, Kovari LC. 2011. Nine crystal structures determine the substrate envelope of the MDR HIV-1 protease. Protein J. 2011 Mar;30(3):173-83. doi: 10.1007/s10930-011-9316-2.


Gupta, D.,  Yedidi R.D., Varghese, S., L.C. Kovari,  and Woster, P.M. 2010. Mechainsm-based inhibitors of the aspartyl protease plasmepsin II as potential antimalarial agents. J Med Chem. 2010 May 27;53(10):4234-47. doi: 10.1021/jm100233b.



Martin P, Vickrey JF, Proteasa G, Jimenez YL, Wawrzak Z, Winters MA, Merigan TC, Kovari LC. 2005. "Wide-open" 1.3 A structure of a multidrug-resistant HIV-1 protease as a drug target. Structure. 2005 Dec;13(12):1887-95.


Winters MA, Kagan RM, Kovari L, Heseltine PN, Merigan TC. 2005. Rare one and two amino acid inserts adjacent to codon 103 of the HIV-1 reverse transcriptase (RT) affect susceptibility to non-nucleoside RT inhibitors. Antivir Ther. 2005;10(2):363-6.


Logsdon BC, Vickrey JF, Martin P, Proteasa G, Koepke JI, Terlecky SR, Wawrzak Z, Winters MA, Merigan TC, Kovari LC. 2004. Crystal structures of a multidrug-resistant human immunodeficiency virus type 1 protease reveal an expanded active-site cavity. J Virol. 2004 Mar;78(6):3123-32.


Purcarea C, Ahuja A, Lu T, Kovari L, Guy HI, Evans DR. 2003. Aquifex aeolicus aspartate transcarbamoylase, an enzyme specialized for the efficient utilization of unstable carbamoyl phosphate at elevated temperature. J Biol Chem. 2003 Dec 26;278(52):52924-34. Epub 2003 Oct 8.


Vickrey JF, Logsdon BC, Proteasa G, Palmer S, Winters MA, Merigan TC, Kovari LC. HIV-1 protease variants from 100-fold drug resistant clinical isolates: expression, purification, and crystallization. Protein Expr Purif. 2003 Mar;28(1):165-72.


Holland-Staley CA, Kovari LC, Golenberg EM, Pobursky KJ, Mayers DL. 2002. Genetic diversity and response to IFN of the NS3 protease gene from clinical strains of the hepatitis C virus. Arch Virol. 2002 Jul;147(7):1385-406.


Winters MA, Coolley KL, Cheng P, Girard YA, Hamdan H, Kovari LC, Merigan TC. 2000. Genotypic, phenotypic, and modeling studies of a deletion in the beta3-beta4 region of the human immunodeficiency virus type 1 reverse transcriptase gene that is associated with resistance to nucleoside reverse transcriptase inhibitors. J Virol. 2000 Nov;74(22):10707-13.



Kovari LC, Momany CA, Miyagi F, Lee S, Campbell S, Vuong B, Vogt VM, Rossmann MG. 1997. Crystals of Rous sarcoma virus capsid protein show a helical arrangement of protein subunits. Virology. 1997 Nov 10;238(1):79-84.


Momany C, Kovari LC, Prongay AJ, Keller W, Gitti RK, Lee BM, Gorbalenya AE, Tong L, McClure J, Ehrlich LS, Summers MF, Carter C, Rossmann MG. 1996. Crystal structure of dimeric HIV-1 capsid protein. Nat Struct Biol. 1996 Sep;3(9):763-70.


Kovari LC, Momany C, Rossmann MG. 1995. The use of antibody fragments for crystallization and structure determinations. Structure. 1995 Dec 15;3(12):1291-3. Review.


Kovari LZ, Fourie M, Park HD, Kovari IA, Van Vuuren HJ, Cooper TG. 1993. Analysis of the inducer-responsive CAR1 upstream activation sequence (UASI) and the factors required for its operation. Yeast. 1993 Aug;9(8):835-45.


Kovari LZ, Kovari I, Cooper TG. 1993. Participation of RAP1 protein in expression of the Saccharomyces cerevisiae arginase (CAR1) gene. J Bacteriol. 1993 Feb;175(4):941-51.


Viljoen M, Kovari LZ, Kovari IA, Park HD, van Vuuren HJ, Cooper TG. 1992. Tripartite structure of the Saccharomyces cerevisiae arginase (CAR1) gene inducer-responsive upstream activation sequence. J Bacteriol. 1992 Nov;174(21):6831-9.


Cooper TG, Kovari L, Sumrada RA, Park HD, Luche RM, Kovari I. 1992. Nitrogen catabolite repression of arginase (CAR1) expression in Saccharomyces cerevisiae is derived from regulated inducer exclusion. J Bacteriol. 1992 Jan;174(1):48-55.


Kovari LZ, Cooper TG. 1991. Participation of ABF-1 protein in expression of the Saccharomyces cerevisiae CAR1 gene. J Bacteriol. 1991 Oct;173(20):6332-8.


Kovari L, Sumrada R, Kovari I, Cooper TG. 1990. Multiple positive and negative cis-acting elements mediate induced arginase (CAR1) gene expression in Saccharomyces cerevisiae. Mol Cell Biol. 1990 Oct;10(10):5087-97.

Dr. Ladislau C. Kovari Wayne State University School of Medicine Dept. of Biochemistry and Molecular Biology 540 E. Canfield Avenue Detroit, MI 48201
E-mail: Tel: 313-577-0296 (lab) Tel: 313-993-1335 (office) Fax: 313-577-2765